Journal: Cerebral Cortex (New York, NY)

Article Title: Neuropilin 1-Sema Signaling Regulates Crossing of Cingulate Pioneering Axons during Development of the Corpus Callosum

doi: 10.1093/cercor/bhp027

Figure Lengend Snippet: Sema3C attracts cingulate cortex neurites in vitro. Cingulate cortex explants grown next to mock-transfected cell blocks (A) or Sema3C-expressing cell blocks (B) for 48 h. Explants have been stained with antineuronal-specific βIII tubulin to visualize neurites. There is no significant difference in neurite outgrowth from explants from either group (C). However, the GR (defined as [(proximal neurite pixels − distal neurite pixels)/(total neurite pixels)) was significantly greater in explants grown next to Sema3C-expressing cell blocks in comparison to controls (*P < 0.0005), indicating that Sema3C acts as an attractant for neurites from cingulate cortex explants. The arrows in A and B indicate the direction of the cell block relative to the explant. Scale bar: 200 μm.

Article Snippet: For the Sema3C culture assay, the primary antibody used was antineuronal-specific βIII tubulin (TuJ-1 clone, R&D Systems, Minneapolis, MN; 1/1000).

Techniques: In Vitro, Transfection, Expressing, Staining, Comparison, Blocking Assay